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Improved stabilization of microencapsulated Cathepsin B in harsh conditions by Swati Sharma; Ashwani Mittal; V.K. Gupta; Hari Singh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A cysteine protease Cathepsin B purified from goat brain was microencapsulated within the CM-cellulose-Ca-alginate membrane having a liquid core in it. The activity yield was maximum under the optimum conditions of immobilization mainly at 4% CM-cellulose, 1% CaCl 2 and 3% alginate concentrations. The encapsulated form of enzyme could retain ∼60% of its original activity during its reusability for more than five successive batch reactions. The physico-chemical characteristics for free and immobilized Cathepsin B were also compared. The optimum pH and temperature of free and immobilized enzyme were 6.0 and 50 • C, and 6.0-6.5 and 55 • C, respectively. The K m value (1.52 mM) for the immobilized enzyme was one and half-fold higher than for the soluble enzyme. The pH and thermal stability as well as organic solvent stability were improved significantly after microencapsulation may make it useful for catalyzing some organic reactions like trans-esterification and trans-amidation.

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Author

Swati Sharma; Ashwani Mittal; V.K. Gupta; Hari Singh

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)