Can I read The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture on EtoBox?

The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture by Jui-Chih Chang; Shan-hui Hsu; David C. Chen is a Engineering article available to read on EtoBox.

What is The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture about?

The dynamic regulation of integrin-binding peptides is crucial for chondrogenic differentiation. Here, we revealed the feasibility for flexible modification of RGD by embedding a large molecular weight and slightly charged (isoelectric point, 6-6.25) RGD-chimeric protein (CBD-RGD) with cellulose-binding domain (CBD) in three dimensional (3D) alginate beads to evaluate the chondrogenesis of adipose-derived adult stem cells (ADAS). The binding of CBD-RGD with cells and its diffusion from alginate beads were studied on fluorescein isothiocyanate (FITC)-conjugated CBD-RGD. The increases in gene expression (Sox9, Aggrecan, fibronectin and collagen II), accumulation of chondrogenic matrices and decrease of collagen X gene expression during TGF-beta3 induction were only observed for those beads containing 10mg/g CBD-RGD initially, with 20.18+/-0.73% of that released in a week. The contrary was observed for beads with CBD-RGD 20mg/g initially and having higher persistence (only 8.6+/-2.17% released in a week). The 10mg/g CBD-RGD-mediated enhancement was demonstrated via the activation of integrin alpha5 and beta1-dependent pathway, and especially related to the upregulation of Sox9 gene an

Who reads The promotion of chondrogenesis in adipose-derived adult stem cells by an RGD-chimeric protein in 3D alginate culture?

Author

Jui-Chih Chang; Shan-hui Hsu; David C. Chen

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)

Published

2009

Language

EN

Field

Engineering (Physical Sciences)