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Quantitative determination of hydroxy fatty acids as an indicator of in vivo lipid peroxidation: Gas chromatography-mass spectrometry methods by David W. Thomas; Frederik J.G.M. van Kuijk; E.A. Dratz; Robert J. Stephens is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Quantitative determination of hydroxy fatty acids as an indicator of in vivo lipid peroxidation: Gas chromatography-mass spectrometry methods about?

A new method has been developed for the quantitation of lipid peroxidation products by gas chromatography-mass spectrometry. An important advantage over existing gas chromatography-mass spectrometry methods is the elimination of autoxidation during sample preparation. The sensitivity is sufficient to permit measurement of lipid peroxidation products under normal physiological conditions on as little as 1 mg of tissue. Lipids from whole tissue samples or cell preparations are reduced by catalytic hydrogenation during extraction. The hydrogenation stabilizes the compounds by saturating the double bonds and reducing the hydroperoxides to hydroxy derivatives. The saturated lipids are then saponified and the resulting fatty acids are converted to pentafluorobenzyl esters. Hydroxy fatty acids are further converted to trimethylsilyl ether derivatives. Quantitation is accomplished by negative ion chemical ionization gas chromatography-mass spectrometry, using deuterated internal standards. Specific products from polyunsaturated fatty acids can be quantitated, and the method differentiates between products produced by free-radical and photooxidation mechanisms. Increased levels of lipid per

Who reads Quantitative determination of hydroxy fatty acids as an indicator of in vivo lipid peroxidation: Gas chromatography-mass spectrometry methods?

Author

David W. Thomas; Frederik J.G.M. van Kuijk; E.A. Dratz; Robert J. Stephens

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)