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Can I read The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target on EtoBox?
The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target by Gergely Rona; Andras Zeke; Bearach Miwatani-Minter; Maren de Vries; Ramanjit Kaur; Austin Schinlever; Sheena Faye Garcia; Hailey V. Goldberg; Hui Wang; Thomas R. Hinds; Fabrice Bailly; Ning Zheng; Philippe Cotelle; Didier Desmaële; Nathaniel R. Landau; Meike Dittmann; Michele Pagano is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target about?
## Abstract The risk of zoonotic coronavirus spillover into the human population, as highlighted by the SARS-CoV-2 pandemic, demands the development of pan-coronavirus antivirals. The efficacy of existing antiviral ribonucleoside/ribonucleotide analogs, such as remdesivir, is decreased by the viral proofreading exonuclease NSP14-NSP10 complex. Here, using a novel assay and in silico modeling and screening, we identified NSP14-NSP10 inhibitors that increase remdesivir’s potency. A model compound, sofalcone, both inhibits the exonuclease activity of SARS-CoV-2, SARS-CoV, and MERS-CoV in vitro, and synergistically enhances the antiviral effect of remdesivir, suppressing the replication of SARS-CoV-2 and the related human coronavirus OC43. The validation of top hits from our primary screenings using cellular systems provides proof-of-concept for the NSP14 complex as a therapeutic target.
Who reads The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gergely Rona; Andras Zeke; Bearach Miwatani-Minter; Maren de Vries; Ramanjit Kaur; Austin Schinlever; Sheena Faye Garcia; Hailey V. Goldberg; Hui Wang; Thomas R. Hinds; Fabrice Bailly; Ning Zheng; Philippe Cotelle; Didier Desmaële; Nathaniel R. Landau; Meike Dittmann; Michele Pagano
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)