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Expression of the chemokine PARC mRNA in bronchoalveolar cells of patients with sarcoidosis by Frantisek Mrazek; Veronika Sekerova; Jiri Drabek; Vitezslav Kolek; Roland M du Bois; Martin Petrek is a Immunology and Microbiology article available to read on EtoBox.
What is Expression of the chemokine PARC mRNA in bronchoalveolar cells of patients with sarcoidosis about?
## Background: Chemotactic cytokines (chemokines) have been recently implicated in the pathogenesis of interstitial lung diseases. a novel chemokine called pulmonary and activation-regulated chemokine (parc/ccl18), which attracts lymphocytes in vitro, has been detected in the human lung. we have, therefore, investigated parc mrna expression in bronchoalveolar lavage fluid (balf) cells of patients with pulmonary sarcoidosis-a disease characterised by a lymphocytic infiltrate. further, because several immunomodulators are used in the treatment of sarcoidosis, we have determined the effects of selected drugs on parc mrna expression in vitro. ## Subjects and methods: Balf cells were obtained by standard bronchoalveolar lavage (bal) from 30 patients with pulmonary sarcoidosis (s) and 16 control subjects (c). balf cells from seven subjects were cultured in the presence of dexamethasone (dx), cyclosporin a (cya) and pentoxifylline (px). parc mrna expression was semiquantitated by reverse-transcription polymerase chain reaction (rt-pcr) using normalisation to the expression of the beta-actin gene. ## Results: Parc mrna transcripts were detected in 87% of all investigated balf samples. the
Who reads Expression of the chemokine PARC mRNA in bronchoalveolar cells of patients with sarcoidosis?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Frantisek Mrazek; Veronika Sekerova; Jiri Drabek; Vitezslav Kolek; Roland M du Bois; Martin Petrek
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)
- Published
- 2002
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)