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Leukemia inhibitory factor induces an antiapoptotic response in oligodendrocytes through Akt-phosphorylation and up-regulation of 14-3-3 by Helena Slaets; Debora Dumont; Joris Vanderlocht; Jean-Paul Noben; Pierre Leprince; Johan Robben; Jerome Hendriks; Piet Stinissen; Niels Hellings is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Leukemia inhibitory factor induces an antiapoptotic response in oligodendrocytes through Akt-phosphorylation and up-regulation of 14-3-3 about?
## Abstract Leukemia inhibitory factor (LIF) promotes the survival of oligodendrocytes (OLG) both __in vitro__ and in an animal model of multiple sclerosis. Here, we show that LIF protects mature rat OLG cultures selectively against the combined insult of the proinflammatory cytokines interferon‐γ and tumor necrosis factor‐α, but it does not protect against oxidative stress nor against staurosporine induced apoptosis. We further demonstrate that LIF activates the janus kinase (JAK)/signal transducer and activator of transcription 3 (STAT3) and the phosphatidylinositol 3 kinase/Akt pathway in mature OLG. We show that LIF protection is independent of suppressors of cytokine signaling and Bcl‐2 mRNA expression levels. To gain further insight into the protective mechanism, a quantitative proteomic approach (DIGE) was applied to identify differentially expressed proteins in LIF‐treated OLG. Our results indicate that LIF induces a shift in the cellular machinery toward a prosurvival execution program, illustrated by an enhanced expression of isoforms of the antiapoptotic molecule 14‐3‐3. These data provide further insight into the mechanisms of LIF‐mediated protection of mature OLGs.
Who reads Leukemia inhibitory factor induces an antiapoptotic response in oligodendrocytes through Akt-phosphorylation and up-regulation of 14-3-3?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Helena Slaets; Debora Dumont; Joris Vanderlocht; Jean-Paul Noben; Pierre Leprince; Johan Robben; Jerome Hendriks; Piet Stinissen; Niels Hellings
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)