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Combination of Suberoylanilide Hydroxamic Acid with Heavy Ion Therapy Shows Promising Effects in Infantile Sarcoma Cell Lines by Susanne Oertel; Markus Thiemann; Karsten Richter; Klaus-J Weber; Peter E Huber; Ramon Lopez Perez; Stephan Brons; Marc Bischof; Andreas E Kulozik; Volker Ehemann; Jürgen Debus; Claudia Blattmann is a Medicine article available to read on EtoBox.
What is Combination of Suberoylanilide Hydroxamic Acid with Heavy Ion Therapy Shows Promising Effects in Infantile Sarcoma Cell Lines about?
## Introduction The pan-HDAC inhibitor (HDACI) suberoylanilide hydroxamic acid (SAHA) has previously shown to be a radio-sensitizer to conventional photon radiotherapy (XRT) in pediatric sarcoma cell lines. Here, we investigate its effect on the response of two sarcoma cell lines and a normal tissue cell line to heavy ion irradiation (HIT). ## Materials and methods Clonogenic assays after different doses of heavy ions were performed. DNA damage and repair were evaluated by measuring γH2AX via flow-cytometry. Apoptosis and cell cycle analysis were also measured via flow cytometry. Protein expression of repair proteins, p53 and p21 were measured using immunoblot analysis. Changes of nuclear architecture after treatment with SAHA and HIT were observed in one of the sarcoma cell lines via light microscopy after staining towards chromatin and γH2AX. ## Results Corresponding with previously reported photon data, SAHA lead to an increase of sensitivity to heavy ions along with an increase of DSB and apoptosis in the two sarcoma cell lines. In contrast, in the osteoblast cell line (hFOB 1.19), the combination of SAHA and HIT showed a significant radio-protective effect. Laser scanning micr
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- Author
- Susanne Oertel; Markus Thiemann; Karsten Richter; Klaus-J Weber; Peter E Huber; Ramon Lopez Perez; Stephan Brons; Marc Bischof; Andreas E Kulozik; Volker Ehemann; Jürgen Debus; Claudia Blattmann
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2006-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1748-717X)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)