Can I read ^1^H,^15^N, and^13^C backbone chemical shift assignments, secondary structure, and magnesium-binding characteristics of the bacillus subtilis response regulator, SpoOF, determined by heteronuclear high-resolution NMR on EtoBox?

^1^H,^15^N, and^13^C backbone chemical shift assignments, secondary structure, and magnesium-binding characteristics of the bacillus subtilis response regulator, SpoOF, determined by heteronuclear high-resolution NMR by Victoria A. Feher; James W. Zapf; James A. Hoch; Frederick W. Dahlquist; John M. Whiteley; John Cavanagh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is ^1^H,^15^N, and^13^C backbone chemical shift assignments, secondary structure, and magnesium-binding characteristics of the bacillus subtilis response regulator, SpoOF, determined by heteronuclear high-resolution NMR about?

Spo0F, sporulation stage 0 F protein, a 124-residue protein responsible, in part, for regulating the transition of Bacillus subtilis from a vegetative state to a dormant endospore, has been studied by high-resolution NMR. The 1H, 15N, and 13C chemical shift assignments for the backbone residues have been determined from analyses of 3D spectra, 15N TOCSY-HSQC, 15N NOESY-HSQC, HNCA, and HN(CO)CA. Assignments for many sidechain proton resonances are also reported. The secondary structure, inferred from short- and medium-range NOEs, 3JHN alpha coupling constants, and hydrogen exchange patterns, define a topology consistent with a doubly wound (alpha/beta)5 fold. Interestingly, comparison of the secondary structure of Spo0F to the structure of the Escherichia coli response regulator, chemotaxis Y protein (CheY) (Volz K, Matsumura P, 1991, J Biol Chem 266:15511-15519; Bruix M et al., 1993, Eur J Biochem 215:573-585), show differences in the relative length of secondary structure elements that map onto a single face of the tertiary structure of CheY. This surface may define a region of binding specificity for response regulators. Magnesium titration of Spo0F, followed by amide chemical sh

Who reads ^1^H,^15^N, and^13^C backbone chemical shift assignments, secondary structure, and magnesium-binding characteristics of the bacillus subtilis response regulator, SpoOF, determined by heteronuclear high-resolution NMR?

Author

Victoria A. Feher; James W. Zapf; James A. Hoch; Frederick W. Dahlquist; John M. Whiteley; John Cavanagh

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)