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This document characterizes a cellobiose 2-epimerase (CE) enzyme from Thermoanaerobacterium saccharolyticum that is capable of producing epilactose from lactose. The recombinant enzyme was expressed in E. coli and purified using a His-tag. It has optimal activity at pH 7.0 and 60°C and is metal independent. Kinetic parameters were determined for the enzyme

Author

Kukymov

Language

EN