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Observations on Assays of Urinary Pregnanediol, Pregnanetriol and Pregnanetriolone by Gas-liquid Chromatography by O.J. Lucis; G.T. Cummings; Y.H. Tsay; B. Minihan is a Medicine article available to read on EtoBox.

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1. After enzymatic hydrolysis and pre-purification of urine extracts on alumina columns, urinary pregnanediol, pregnanetriol and pregnanetriolone can be separated and quantitated without formation of derivatives by gas-liquid chromatography. 2. Separation of the steroid metabolites is achieved by chromatography at 235 ° C on a glass column containing Diatoport-S -F 3.8% SE-30. 3. Cholestane serves as internal standard for chromatography of pregnanediol. Pure pregnanediol is useful as internal standard for separation of pregnanetriol and pregnanetriolone. 4. In 12 normally menstruating females pregnanediol excretion in the follicular phase ranged from 0.2-1.5 mg/24 hrs and in the luteal phase from 0.8-4.1 mg/24 hrs. Seven healthy males excreted pregnanediol in a range from 0.3 to 1.0 mg/24 hrs. 5. Pregnanetriolone was detected in the urine only in patients with congenital adrenal hyperplasia. 6. Pregnanetriol values obtained by gas-liquid chromatography have been compared with colorimetric assay. THE CYCLIC CHANGES IN THE URINARY EXCRETION OF PREGNANEDIOL (5#-pregnane-3a,20a-diol) in non-pregnant subjects serves as a useful index for the detection of ovulation and for an assessment

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Author

O.J. Lucis; G.T. Cummings; Y.H. Tsay; B. Minihan

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-9120)

Published

1968

Language

EN

Field

Medicine (Health Sciences)