Can I read Characterization and discrimination of citrus canker causing pathotypes in citrus trees using RAPD and ISSR markers on EtoBox?

Characterization and discrimination of citrus canker causing pathotypes in citrus trees using RAPD and ISSR markers by Zohreh Ebrahimi; Rasool Rezaei; Asad Masoumi-Asl; Fariba Ghaderi is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Characterization and discrimination of citrus canker causing pathotypes in citrus trees using RAPD and ISSR markers about?

The present study was conducted to differentiate pathotypes of Xanthomonas citri subsp. citri (Xcc) the causal agent of citrus canker in Iran. A collection of 29 strains were isolated from infected plant parts. In order to investigate the genetic diversity of bacterial isolates, RAPD and ISSR markers were used and discrimination power of these markers for the pathotype detection was assessed. Based on the amplified fragments of the RAPD, the dendrogram divided the strains into three main groups. In the first group, there were eight strains of both pathotypes A and A\*, and all strains of A\* pathotype were categorized in this group. Strains of pathotype A formed the second and third groups. Based on the results of the ISSR amplified fragments, the dendrogram classified the strains into three main groups. The resultant grouping was indicative of placement of A strains in one group, and in all groups, both A and A\* pathotypes were grouped together. The electrophoresis patterns of amplified fragments with RAPD primers revealed high specificity of this marker in pathotype differentiation.

Who reads Characterization and discrimination of citrus canker causing pathotypes in citrus trees using RAPD and ISSR markers?

Author

Zohreh Ebrahimi; Rasool Rezaei; Asad Masoumi-Asl; Fariba Ghaderi

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)