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Oral Pathogenic Bacteria-Inducing Neurodegenerative Microgliosis in Human Neural Cell Platform by Tran Thi Ai Tran; You Jung Kang; Hyun-Kyoung Kim; Hyung-Ryong Kim; Hansang Cho is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Oral Pathogenic Bacteria-Inducing Neurodegenerative Microgliosis in Human Neural Cell Platform about?

__Porphyromonas gingivalis__ is a gram-negative bacterium found in the human oral cavity and is responsible for the development of chronic periodontitis as well as neurological diseases, including Alzheimer’s disease (AD). Given the significance of the roles of __P. gingivalis__ in AD pathogenesis, it is critical to understand the underlying mechanisms of __P. gingivalis__-driven neuroinflammation and their contribution to neurodegeneration. Herein, we hypothesize that __P. gingivalis__ produces secondary metabolites that may cause neurodegeneration through direct or indirect pathways mediated by microglia. To test our hypothesis, we treated human neural cells with bacterial conditioned media on our brain platforms and assessed microgliosis, astrogliosis and neurodegeneration. We found that bacteria-mediated microgliosis induced the production of nitric oxide, which causes neurodegeneration assessed with high pTau level. Our study demonstrated the elevation of detrimental protein mediators, CD86 and iNOS and the production of several pro-inflammatory markers from stimulated microglia. Through inhibition of LPS and succinate dehydrogenase in a bacterial conditioned medium, we showed

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Author

Tran Thi Ai Tran; You Jung Kang; Hyun-Kyoung Kim; Hyung-Ryong Kim; Hansang Cho

Publisher

MDPI AG

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)