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Increased proteome coverage for quantitative peptide abundance measurements based upon high performance separations and DREAMS FTICR mass spectrometry by Ljiljana Paša-Tolić; Richard Harkewicz; Gordon A. Anderson; Nikola Tolić; Yufeng Shen; Rui Zhao; Brian Thrall; Christophe Masselon; Richard D. Smith is a Chemistry article available to read on EtoBox.
What is Increased proteome coverage for quantitative peptide abundance measurements based upon high performance separations and DREAMS FTICR mass spectrometry about?
A primary challenge in proteome measurements is to be able to detect, identify, and quantify the extremely complex mixtures of proteins. The relative abundances of interest span at least six orders of magnitude for mammalian proteomes, and this constitutes an intractable challenge for high throughput proteome studies. We have recently described a new approach, Dynamic Range Enhancement Applied to Mass Spectrometry (DREAMS), which is based upon the selective ejection of the most abundant species to expand the dynamic range of Fourier transform ion cyclotron resonanace (FTICR) measurements. The basis of our approach is on-the-fly data-dependent selective ejection of highly abundant species, followed by prolonged accumulation of remaining low-abundance species in a quadrupole external to the FTICR ion trap. Here we report the initial implementation of this approach with high efficiency capillary reverse phase LC separations and high magnetic field electrospray ionization FTICR mass spectrometry for obtaining enhanced coverage in quantitative measurements for mammalian proteomes. We describe the analysis of a sample derived from a tryptic digest of proteins from mouse B16 cells culture
Who reads Increased proteome coverage for quantitative peptide abundance measurements based upon high performance separations and DREAMS FTICR mass spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Ljiljana Paša-Tolić; Richard Harkewicz; Gordon A. Anderson; Nikola Tolić; Yufeng Shen; Rui Zhao; Brian Thrall; Christophe Masselon; Richard D. Smith
- Publisher
- Elsevier Science; Springer-Verlag; Springer New York LLC; American Chemical Society (ACS); Springer Science and Business Media LLC; Elsevier BV (ISSN 1044-0305)
- Published
- 2002
- Language
- EN
- Field
- Chemistry (Physical Sciences)