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Affinity of the monoclonal antibody M1 directed against the FLAG peptide by A. Einhauer; A. Jungbauer is a Chemistry article available to read on EtoBox.
What is Affinity of the monoclonal antibody M1 directed against the FLAG peptide about?
The FLAG (Sigma, St. Louis, MO, USA) peptide is a frequently used hydrophilic and immunogenic fusion tag which was specifically designed to facilitate rapid purification by immunoaffinity chromatography. The monoclonal antibody M1 recognizes the free N-terminus of the peptide tag in a calcium dependent manner. Dissociation of the complex can be performed by the addition of chelating agents such as EDTA. This effect can be exploited for immunoaffinity purification of FLAG-tagged fusion proteins. Kinetic information obtained from monitoring interactions in real-time measurement (Biacore 2000) using surface plasmon resonance as detection principle did not show any difference for association and dissociation 3 21 21 23 21 2 1 3 21 rate constants in the presence (k 53.03?10 M s k 51.25?10 s ) and in the absence of Ca (k 53.59?10 M a , d a 21 23 21 s , k 51.16?10 s ). These findings corroborate the reports from Mol. Immunol. 33 (1996) 601-608 describing similar d binding analyzed by enzyme-linked immunosorbent assay experiments. These investigations are in contrast to the observations in immunoaffinity chromatography with immobilized anti-FLAG antibody M1.
Who reads Affinity of the monoclonal antibody M1 directed against the FLAG peptide?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- A. Einhauer; A. Jungbauer
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2001
- Language
- EN
- Field
- Chemistry (Physical Sciences)