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What is Buffer Saturated Phenol for DNA Extraction about?

This document provides instructions for preparing buffer saturated phenol for DNA extraction. Phenol is heated to 65°C and 8-hydroxyquinoline is added to prevent oxidation. Equal volumes of 0.5M Tris-Cl buffer at pH 8.0 are added and mixed, then the phases are separated. This is repeated twice with the same buffer and once with 0.1M Tris-Cl buffer at pH 8.0 until the aqueous phase reaches pH 7.8. The prepared buffer saturated phenol can be stored at 4°C for up to 1 month.

Author

Yorlady

Language

EN