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Detailed Analysis of Reactive Oxygen Species Induced by Visible Light in Various Cell Types by Ronit Lavi; Asher Shainberg; Vladimir Shneyvays; Elicheva Hochauser; Ahuva Isaac; Tova Zinman; Harry Friedmann; Rachel Lubart is a Medicine article available to read on EtoBox.
What is Detailed Analysis of Reactive Oxygen Species Induced by Visible Light in Various Cell Types about?
## Abstract ## Background and Objective Light in the visible and near infrared region stimulates various cellular processes, and thus has been used for therapeutic purposes. One of the proposed mechanisms is based on cellular production of reactive oxygen species (ROS) in response to illumination. In the present study, we followed visible light (VL)‐induced hydroxyl radicals in various cell types and cellular sites using the electron paramagnetic resonance (EPR) spin‐trapping technique. ## Materials and Methods Fibroblasts, sperm cells, cardiomyocytes, and skeletal muscle cells were irradiated with broadband (400–800 nm) VL. To detect ROS, the EPR spin‐trapping technique coupled with the spin‐traps 5,5‐dimethyl pyrroline‐__N__‐oxide (DMPO) or 5‐(diethoxyphosphoryl)‐5‐methyl‐1‐pyrroline‐__N__‐oxide (DEPMPO) were used. To investigate the cellular sites of ROS formation, the cell‐permeable molecule, isopropanol, or the nonpermeable proteins, bovine serum albumin (BSA) and superoxide dismutase (SOD), were introduced to the cells before irradiation. ROS production in mitochondria was measured using the fluorescent probe, MitoTracker Red (MTR). ## Results and Conclusions The concentratio
Who reads Detailed Analysis of Reactive Oxygen Species Induced by Visible Light in Various Cell Types?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Ronit Lavi; Asher Shainberg; Vladimir Shneyvays; Elicheva Hochauser; Ahuva Isaac; Tova Zinman; Harry Friedmann; Rachel Lubart
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0196-8092)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)