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This study aimed to construct chimeric gene cassettes containing the CaMV 35S promoter or OsAER1 gene promoter fused to the GUS reporter gene, and to develop an efficient method for Agrobacterium tumefaciens-mediated transient transformation of tobacco seedlings. The constructs were transformed into tobacco seedlings using different bacterial densities and vacuum treatments. Expression of the GUS gene was observed in different tobacco tissues depending on the promoter used, demonstrating that transient tran

Author

Eva

Language

EN