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Hypermethylation of the APC promoter but lack of APC mutations in myxoid/round-cell liposarcoma by Sonja Sievers; Carla Fritzsch; Marcus Lehnhardt; Susanne Zahn; Nadine Kutzner; Cornelius Kuhnen; Oliver Müller is a Medicine article available to read on EtoBox.

What is Hypermethylation of the APC promoter but lack of APC mutations in myxoid/round-cell liposarcoma about?

## Abstract The adenomatous polyposis coli (APC) protein is a key component of the WNT signalling pathway wherein it acts as a scaffolding protein in controlling the level of the proto‐oncoprotein β‐catenin. Although __APC__ has been shown to be genetically or epigenetically inactivated in a variety of carcinomas, little is known about its role in sarcoma. Liposarcomas (LPSs) are the second most common soft tissue sarcoma in adults. Despite different histology and malignancy, the myxoid and round‐cell LPSs belong to one tumour entity characterized by a specific chromosomal translocation. We assessed the extent of genetic and epigenetic inactivation of the __APC__ gene in myxoid/round‐cell LPS. Sequencing of the mutation cluster region, the protein truncation test and a loss of heterozygosity (LOH) analysis did not reveal any genetic alterations of the __APC__ gene in all of the liposarcoma samples. Methylation of the __APC__ promoter was detected by methylation‐specific PCR in 9 of 20 (45%) tumours. Analysis of __APC__ expression by semiquantitative RT‐PCR in a subset of the samples demonstrated that tumours with a methylated __APC__ promoter showed a downregulation of the __APC__

Who reads Hypermethylation of the APC promoter but lack of APC mutations in myxoid/round-cell liposarcoma?

Author

Sonja Sievers; Carla Fritzsch; Marcus Lehnhardt; Susanne Zahn; Nadine Kutzner; Cornelius Kuhnen; Oliver Müller

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2006

Language

EN

Field

Medicine (Health Sciences)