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Silencing of Tissue Factor Pathway Inhibitor-2 Gene in Malignant Melanomas by Yoshimasa Nobeyama; Eriko Okochi-Takada; Junichi Furuta; Yohei Miyagi; Kanako Kikuchi; Akifumi Yamamoto; Yukihiro Nakanishi; Hidemi Nakagawa; Toshikazu Ushijima is a Medicine article available to read on EtoBox.

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## Abstract To identify tumor‐suppressor genes inactivated by aberrant methylation of promoter CpG islands (CGIs) in human malignant melanomas, genes upregulated by treatment of cells with a demethylating agent, 5‐aza‐2′‐deoxycytidine (5‐aza‐dC), were searched for using oligonucleotide microarrays in melanoma cell lines, HMV‐I, MeWo and WM‐115. Seventy‐nine known genes with CGIs were identified as being upregulated (≥16‐fold), and 18 of them had methylation of their putative promoter CGIs in 1 or more of 8 melanoma cell lines. Among the 18 genes, __TFPI‐2,__ which is involved in repression of the invasive potential of malignant melanomas, was further analyzed. Its expression was repressed in a melanoma cell line with its complete methylation, and was restored by 5‐aza‐dC treatment. It was unmethylated in cultured neonatal normal epidermal melanocyte, and was induced by ultraviolet B. In surgical melanoma specimens, __TFPI‐2__ methylation was detected in 5 of 17 metastatic site specimens (29%), while it was not detected in 20 primary site specimens (0%) (__p__ = 0.009). By immunohistochemistry, the 5 specimens with promoter methylation lacked immunoreactivity for TFPI‐2. The results

Who reads Silencing of Tissue Factor Pathway Inhibitor-2 Gene in Malignant Melanomas?

Author

Yoshimasa Nobeyama; Eriko Okochi-Takada; Junichi Furuta; Yohei Miyagi; Kanako Kikuchi; Akifumi Yamamoto; Yukihiro Nakanishi; Hidemi Nakagawa; Toshikazu Ushijima

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2007

Language

EN

Field

Medicine (Health Sciences)