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Identification of Tumor Vascular Antigens by Monoclonal Antibodies Prepared from Rat-tumor-derived Endothelial Cells by Iwao Ohizumi; Shin-ichi Tsunoda; Kenji Taniguchi; Hiroyuki Saito; Keiko Esaki; Keiichi Koizumi; Hiroo Makimoto; Yukiko Wakai; Junji Matsui; Yasuo Tsutsumi; Shinsaku Nakagawa; Naoki Utoguchi; Yoshiyuki Ohsugi; Tadanori Mayumi is a Medicine article available to read on EtoBox.
What is Identification of Tumor Vascular Antigens by Monoclonal Antibodies Prepared from Rat-tumor-derived Endothelial Cells about?
We have reported the isolation and specific in vitro properties of tumor-derived endothelial cells (TEC) from rat KMT-17 fibrosarcomas transplanted into rats. To develop antibodybased tumor vascular targeting therapy for solid tumors, we have generated monoclonal antibodies (MAbs) using passive immunization of outside-out membrane vesicles of rat epididymal-fat-pad-derived capillary endothelial cells (FCEC) followed by active immunization of those of rat TEC. The MAbs produced were screened against TEC and FCEC. Of all cultured hybridomas, 75 (3.3%) of the secreted MAbs preferentially recognized TEC. We selected a total of 7 MAbs which detected antigens highly abundant in TEC, although 5 of the 7 MAbs were weakly positive for FCEC in cell-ELISA and FACS analyses. The antigens recognized by these MAbs, with the exception of MAb TES-7, were present on endothelial cells of tumor blood vessels in KMT-17 fibrosarcoma tissues, as shown by immunohistochemical analysis. Antigens of 40-and 80-kDa were recognized by MAbs TES-1, 7, 17, 21 and 26 and by MAbs TES-23 and 27 respectively. Although the function of these antigens, which are preferentially expressed on rat tumor-derived endothelial
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- Author
- Iwao Ohizumi; Shin-ichi Tsunoda; Kenji Taniguchi; Hiroyuki Saito; Keiko Esaki; Keiichi Koizumi; Hiroo Makimoto; Yukiko Wakai; Junji Matsui; Yasuo Tsutsumi; Shinsaku Nakagawa; Naoki Utoguchi; Yoshiyuki Ohsugi; Tadanori Mayumi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1998
- Language
- EN
- Field
- Medicine (Health Sciences)