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Rapid label-free colorimetric dual-functional aptasensor for β-lactoglobulin detection based on a rational tailoring strategy 1 1 Introduction 1 2 Materials and methods 2 2.1 Reagents 2 2.2 Rational aptamer tailoring protocol 2 2.3 Colorimetric analysis to detect hemin/G4 DNAzyme activity 2 2.4 Circular dichroism 2 3 Results and discussion 2 3.1 Principle of the scheme 2 3.2 Rational tailoring into a flexible, topological dual-functional aptamer 2 3.3 Factors influencing the dual-functionality of the aptamer 3 3.4 Optimization of the aptasensor 4 3.5 Sensitivity of the aptasensor 4 3.6 Selectivity of the aptasensor 5 3.7 Analysis of β-LG in the spiked dairy samples 5 4 Conclusions 6 CRediT authorship contribution statement 6 Declaration of competing interest 6 Acknowledgements 7 Appendix A Supplementary data 7 References 7
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