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Synthesis of Charged Amphipathic Nitroxide Lipid Spin Labels and an Example of Their Application in Membrane Studies by John F.W. Keana; Steven A. Boyd; Debra A. McMillen; Edward M. Bernard; Patricia C. Jost is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Synthesis of Charged Amphipathic Nitroxide Lipid Spin Labels and an Example of Their Application in Membrane Studies about?

The synthesis of a series of amphipathic nitroxide lipid spin labels is reported. Thus, 12-proxylhexadecanol has been converted into the versatile fatty acid spin label 14-proxylstearic acid. This substance was used to prepare 14-proxylstearyltrimethylammonium methanesulfonate, a positively charged label, and 14-proxylstearylmethyl phosphate sodium salt, a negatively charged label. Also prepared in the doxyl series were quaternary ammonium salts derived from 16-doxyl- and 7-doxylstearic acid. The positively charged and negatively charged proxyl labels were used in a preliminary experiment to investigate the role of charge in their interaction with reconstituted cytochrome oxidase. The average binding affinity of the negatively charged label is approximately 2-fold higher than that of the positively charged label at pH 7.4. At pH 5.5 the average relative affinity for negatively charged label is about 3.5-fold higher than that of positively charged label, suggesting that the ionizable group(s) on the protein can interact with the lipid headgroup.

Who reads Synthesis of Charged Amphipathic Nitroxide Lipid Spin Labels and an Example of Their Application in Membrane Studies?

Author

John F.W. Keana; Steven A. Boyd; Debra A. McMillen; Edward M. Bernard; Patricia C. Jost

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-3084)

Published

1982

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)