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Fluorescence Energy Transfer Probes Based on the Guanine Quadruplex Formation for the Fluorometric Detection of Potassium Ion by Satoru Nagatoishi; Takahiko Nojima; Elzbieta Galezowska; Agata Gluszynska; Bernard Juskowiak; Shigeori Takenaka is a Chemistry article available to read on EtoBox.
What is Fluorescence Energy Transfer Probes Based on the Guanine Quadruplex Formation for the Fluorometric Detection of Potassium Ion about?
Dual-labeled oligonucleotide derivative, FAT-0, carrying 6-carboxyfluorescein (FAM) and 6-carboxy-tetramethylrhodamine (TAMRA) labels at 5 -and 3 -termini of thrombin-binding aptamer (TBA) sequence 5 -GGTTGGTGTGGTTGG-3 and its derivatives, FAT-n (n = 3, 5, and 7) were designed and synthesized. FAT-n derivatives contained a T m A spacer (m = 2, 4, and 6, respectively) at 5 -end of TBA sequence. The probes were developed to estimate the spacer effect on FRET efficiency and to identify the best probe for sensing of K + . Circular dichroism (CD), UV-vis absorption, and fluorescence studies revealed that all FAT-n probes could form the intramolecular tetraplex structures after binding K + . Association constants of particular K + /FAT-n complexes were determined using different experimental approaches. Suitability of particular probes for sensitive monitoring of K + in intra-and extracellular conditions was examined and discussed. Calibration graphs of fluorescence ratio were linear in the K + concentration range of 2-10 mM for extracellular conditions showing sensitivity of 1.2% mM -1 K + and for intracellular conditions in the range of 100-200 mM with sensitivity of 0.49% mM -1 K + .
Who reads Fluorescence Energy Transfer Probes Based on the Guanine Quadruplex Formation for the Fluorometric Detection of Potassium Ion?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Satoru Nagatoishi; Takahiko Nojima; Elzbieta Galezowska; Agata Gluszynska; Bernard Juskowiak; Shigeori Takenaka
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0003-2670)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)