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What is IHC Protocol Steps for Paraffin Sections about?

1. The document outlines the protocol for immunohistochemistry (IHC) staining of paraffin embedded tissue sections. 2. The protocol involves deparaffinization of the sections, antigen retrieval by heating the slides in sodium citrate buffer, blocking non-specific binding with serum, incubation with primary and secondary antibodies, development with DAB, counterstaining, dehydration and mounting. 3. Additional protocols are provided for immunofluorescence staining of paraffin and frozen tissue sections whic

Author

Pradeep Patil

Language

EN