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A Surgical Technique for Administering Laser Irradiation to Rat Incisor Dentin by Patricia L. Ling; Algernon C. Karim is a Medicine article available to read on EtoBox.

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With the advent of lasers in the field of dentistry, various methods have been used to expose dentin to laser irradiation. This was done indirectly through the alveolar bone, as well as directly on dentin wafers and on extracted human teeth. However, dentin is a vital tissue and the only way to show the ramifications of laser irradiation is by directly exposing the dentin to this energy source under in vivo conditions. In the present study we describe a highly reproducible method for accomplishing this. Male Sprague Dawley rats were anesthetized and an incision was made at the junction of the attached palatal mucosa and the unattached buccal mucosa parallel to the maxillary bones on both sides. This procedure exposed the underlying bone where a 2 mm square window, at a point 3 mm caudal and parallel to the gingival attachment, was cut out to the depth of the periodontal ligament. The site was flushed with a 0.9% sterile sodium chloride solution and gently dried with gauze. The beam of the laser was focused in the middle of the 2 mm square window and a pre-determined energy level and pulse duration (2 W 50 ms or 10 W 50 ms) were selected. The focal length guide was held at right ang

Who reads A Surgical Technique for Administering Laser Irradiation to Rat Incisor Dentin?

Author

Patricia L. Ling; Algernon C. Karim

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0003-276X)

Published

2000

Language

EN

Field

Medicine (Health Sciences)