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Can I read Metabolism of Catecholamines by Catechol-O-Methyltransferase in Cells Expressing Recombinant Catecholamine Transporters on EtoBox?
Metabolism of Catecholamines by Catechol-O-Methyltransferase in Cells Expressing Recombinant Catecholamine Transporters by Amy J. Eshleman; Emilie Stewart; Anna K. Evenson; John N. Mason; Randy D. Blakely; Aaron Janowsky; Kim A. Neve is a Neuroscience article available to read on EtoBox.
What is Metabolism of Catecholamines by Catechol-O-Methyltransferase in Cells Expressing Recombinant Catecholamine Transporters about?
Abstract: To determine if catechol‐__O__‐methyltransferase (COMT) metabolizes catecholamines within cell lines used for heterologous expression of plasmalemmal transporters and alters the measured characteristics of ^3^H‐substrate transport, the uptake of monoamine transporter substrates was assessed in three cell lines (C6 glioma, L‐M fibroblast, and HEK293 cells) that had been transfected with the recombinant human transporters. Uptake and cellular retention of ^3^H‐catecholamines was increased by up to fourfold by two COMT inhibitors, tropolone and Ro 41‐0960, with potencies similar to those for inhibition of COMT activity, whereas the uptake of two transporter substrates that are not substrates for COMT, [^3^H]serotonin and [^3^H]MPP^+^, was unaffected. Direct measurement of monoamine substrates by HPLC confirmed that tropolone (1 m__M__) increased the retention of the catecholamines dopamine and norepinephrine, but not the retention of serotonin in HEK293 cells. Saturation analysis of the uptake of [^3^H]dopamine by C6 cells expressing the dopamine transporter demonstrated that tropolone (1 m__M__) decreased the apparent __K__~m~ of transport from 0.61 μ__M__ to 0.34 μ__M__ wi
Who reads Metabolism of Catecholamines by Catechol-O-Methyltransferase in Cells Expressing Recombinant Catecholamine Transporters?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Amy J. Eshleman; Emilie Stewart; Anna K. Evenson; John N. Mason; Randy D. Blakely; Aaron Janowsky; Kim A. Neve
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 1997
- Language
- EN
- Field
- Neuroscience (Life Sciences)