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Real-Time Parallel Artificial Membrane Permeability Assay Based on Supramolecular Fluorescent Artificial Receptors by He, Suhang; Zhiti, Anxhela; Barba-Bon, Andrea; Hennig, Andreas; Nau, Werner M. is a Engineering article available to read on EtoBox.

What is Real-Time Parallel Artificial Membrane Permeability Assay Based on Supramolecular Fluorescent Artificial Receptors about?

Parallel artificial membrane permeability assay (PAMPA) is a screening tool for the evaluation of drug permeability across various biological membrane systems in a microplate format. In PAMPA, a drug candidate is allowed to pass through the lipid layer of a particular well during an incubation period of, typically, 10-16 h. In a second step, the samples of each well are transferred to a UV-Vis-compatible microplate and optically measured (applicable only to analytes with sufficient absorbance) or sampled by mass-spectrometric analysis. The required incubation period, sample transfer, and detection methods jointly limit the scalability of PAMPA to high-throughput screening format. We introduce a modification of the PAMPA method that allows direct fluorescence detection, without sample transfer, in real time (RT-PAMPA). The method employs the use of a fluorescent artificial receptor (FAR), composed of a macrocycle in combination with an encapsulated fluorescent dye, administered in the acceptor chamber of conventional PAMPA microplates. Because the detection principle relies on the molecular recognition of an analyte by the receptor and the associated fluorescence response, concentra

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Author

He, Suhang; Zhiti, Anxhela; Barba-Bon, Andrea; Hennig, Andreas; Nau, Werner M.

Publisher

Frontiers Media SA; Frontiers Media S.A.; Lausanne: Frontiers Media S.A., 2013- (ISSN 2296-2646)

Published

2020

Language

EN

Field

Engineering (Physical Sciences)