Can I read Thidiazuron-induced in vitro shoot formation from roots of intact seedlings of Albizzia julibrissin on EtoBox?

Thidiazuron-induced in vitro shoot formation from roots of intact seedlings of Albizzia julibrissin by Daksha Sankhla; Tim D. Davis; N. Sankhla is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Thidiazuron-induced in vitro shoot formation from roots of intact seedlings of Albizzia julibrissin about?

Seedlings of silktree (Albizzia julibrissin Durrazz .) were grown in vitro on MS-media containing B5 vitamins, 3% sucrose, 0.25% phytagel and various concentrations (0 .1-10 μM) of thidiazuron (TDZ) . Addition of TDZ to the culture medium greatly reduced shoot and root elongation but did not influence shoot production from the cotyledonary node or apex . Within 8-10 days the seedling roots split open, formed large masses of callus, and developed green patches which eventually grew into normal shoots while still within the culture medium containing TDZ at 0.1-1 .0 μM . Such callus and shoot formation did not occur in control cultures lacking TDZ . At higher TDZ concentrations (2 .5-10 ,tiM), the green patches formed in the callus did not further develop into shoots . Addition of other cytokinins (kinetin, benzylaminopurine, zeatin) to the culture medium also induced some shoot formation from the roots, but higher concentrations than TDZ were required to induce regeneration . Isopentenyladenine failed to induced shoot formation . Following excision and transfer to MS media with or without 4 .9 pM IBA, the shoots induced by kinetin or benzylaminopurine rooted 4-7 days earlier than tho

Who reads Thidiazuron-induced in vitro shoot formation from roots of intact seedlings of Albizzia julibrissin?

Author

Daksha Sankhla; Tim D. Davis; N. Sankhla

Publisher

Springer Netherlands; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0167-6903)

Published

1994

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)