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Five distinct ester hydrolases have been characterized in guinea-pig epidermis, including carboxylic esterase, acid phosphatase, pyrophosphatase, and arylsulphatases A and B. Their properties are consistent with lysosomal enzymes. The activities of these ester hydrolases were characterized and quantified using fluorogenic substrates. Kinetic studies showed linear progress curves for up to 2 hours and Michaelis-Menten kinetics for carboxylic esterase, acid phosphatase, and pyrophosphatase. Arylsulphatase dis

Author

Yuneke Bahri

Language

EN