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A Desaturase‐Like Enzyme Catalyzes Oxazole Formation in Pseudomonas Indolyloxazole Alkaloids by Alexander O. Brachmann; Silke I. Probst; Joel Rüthi; Darya Dudko; Helge B. Bode; Jörn Piel is a Chemistry article available to read on EtoBox.
What is A Desaturase‐Like Enzyme Catalyzes Oxazole Formation in Pseudomonas Indolyloxazole Alkaloids about?
## Abstract Indolyloxazole alkaloids occur in diverse micro‐ and macroorganisms and exhibit a wide range of pharmacological activities. Despite their ubiquitous occurrence and simple structures, the biosynthetic pathway remained unknown. Here, we used transposon mutagenesis in the labradorin producer Pseudomonas entomophila to identify a cryptic biosynthetic locus encoding an N‐acyltransferase and a non‐heme diiron desaturase‐like enzyme. Heterologous expression in E. coli demonstrates that both enzymes are sufficient to produce indolyloxazoles. Probing their function in stable‐isotope feeding experiments, we provide evidence for an unusual desaturase mechanism that generates the oxazole by decarboxylative cyclization.
Who reads A Desaturase‐Like Enzyme Catalyzes Oxazole Formation in Pseudomonas Indolyloxazole Alkaloids?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Alexander O. Brachmann; Silke I. Probst; Joel Rüthi; Darya Dudko; Helge B. Bode; Jörn Piel
- Publisher
- Wiley
- Published
- 2021
- Language
- EN
- Field
- Chemistry (Physical Sciences)