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Can I read Carbamylation of Erythrocyte Membrane Aminophospholipids: an in Vitro and in Vivo Study on EtoBox?
Carbamylation of Erythrocyte Membrane Aminophospholipids: an in Vitro and in Vivo Study by Daniel J. Trepanier; Roger J. Thibert is a Medicine article available to read on EtoBox.
What is Carbamylation of Erythrocyte Membrane Aminophospholipids: an in Vitro and in Vivo Study about?
Objectives: To study the binding of cyanate to erythrocyte membrane aminophospholipids in vitro, and to investigate whether carbamylated aminophospholipids can be detected in the plasma membrane of native erythrocytes. ## Design and Methods: For in vitro studies, the lipid components of 14C-carbamylated erythrocyte membranes were resolved by thinlayer chromatography (TLC). The covalent incorporation of cyanate was visualized by autoradiography and quantitated by phosphorus analysis. For the in vivo studies, phospholipid headgroups were enzymatically hydrolyzed by phospholipase D and subsequently reacted with diacetyl monoxime. Results: Both phosphatidylethanolamine (PE) and phosphatidylserine (PS) were covalently modified by [14C] cyanate; incorporating 15.76 ± 0.09 and 13.34 ± 0.81 mol%, respectively, following a 15-h incubation. Carbamylated PE (carb-PE) was resolved with PE by TLC in a solvent system consisting of chloroform~methanol~ammonia (65/35/5, v/v/v). Treatment of native erythrocyte membrane lipid micelles with phospholipase D, followed by reaction with diacetyl monoxime, suggests the presence of intrinsic carb-PE (2.85 ± 0.65 percent of the total PE). Conclusions: Carba
Who reads Carbamylation of Erythrocyte Membrane Aminophospholipids: an in Vitro and in Vivo Study?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Daniel J. Trepanier; Roger J. Thibert
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-9120)
- Published
- 1996
- Language
- EN
- Field
- Medicine (Health Sciences)