Can I read Fluorescence Plate Reader for Kinetic Analysis on EtoBox?

Fluorescence Plate Reader for Kinetic Analysis by Cat Yang is a document available to read on EtoBox.

What is Fluorescence Plate Reader for Kinetic Analysis about?

This article presents a method for determining kinetic parameters of fluorescence resonance energy transfer (FRET) substrates using a fluorescence plate reader, incorporating an inner filter effect correction for accurate measurements. The study reports kinetic values for HCV NS3 protease substrates, including K_m and k_cat/K_m, which align closely with HPLC-determined values. The methodology allows for rapid assessment of enzyme kinetics and inhibitor mechanisms, demonstrating the effectiveness of the inne

Author

Cat Yang

Language

EN