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Sequence polymorphism in the ribosomal DNA internal transcribed spacers differs among Theileria species by Münir Aktas; Kylie G. Bendele; Kürsat Altay; Nazir Dumanli; Masayoshi Tsuji; Patricia J. Holman is a Immunology and Microbiology article available to read on EtoBox.
What is Sequence polymorphism in the ribosomal DNA internal transcribed spacers differs among Theileria species about?
The genomic region spanning the two ribosomal RNA internal transcribed spacers (ITS1 and ITS2) and the 5.8S rRNA gene was cloned and sequenced from sixteen Theileria isolates. Each Theileria species possessed ITS1 and ITS2 of unique size(s) and species specific nucleotide sequences. Varying degrees of ITS1 and ITS2 intra-and inter-species sequence polymorphism were found among ruminant Theileria species. The spacers were most polymorphic in the agent of tropical theileriosis, Theileria annulata, and were more conserved in two benign species, Theileria buffeli and Theileria sergenti Chitose. Phylogenetic analysis of the rDNA ITS1-5.8S rRNA gene-ITS2 region clearly separated each taxon, placing them in three clusters. One held T. annulata, Theileria parva, and Theileria mutans, with the latter two most closely related. The second held T. sergenti Ikeda, T. sergenti Chitose, and T. buffeli, with the latter two most closely related. The third cluster held the Theileria ovis isolates.
Who reads Sequence polymorphism in the ribosomal DNA internal transcribed spacers differs among Theileria species?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Münir Aktas; Kylie G. Bendele; Kürsat Altay; Nazir Dumanli; Masayoshi Tsuji; Patricia J. Holman
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0304-4017)
- Published
- 2007
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)