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Enhancement of Aquaporin-3 by Vasoactive Intestinal Polypeptide in a Human Colonic Epithelial Cell Line by AKIHIKO ITOH; TOMOYUKI TSUJIKAWA; YOSHIHIDE FUJIYAMA; TADAO BAMBA is a Medicine article available to read on EtoBox.

What is Enhancement of Aquaporin-3 by Vasoactive Intestinal Polypeptide in a Human Colonic Epithelial Cell Line about?

## Abstract **Background:** Vasoactive intestinal polypeptide (VIP) plays an important role in water transport in the intestine. Several specialized channels termed aquaporins (AQP) facilitate water transport in the gastrointestinal tract. Aquaporin‐3 localizes to epithelial cells in the human small intestine and colon. However, the regulatory mechanisms underlying the functions of AQP3 remain unclear. To characterize the regulation of AQP3 expression by VIP, we studied messenger (m)RNA expression, protein expression and DNA binding activity in a human colonic epithelial cell line, HT‐29. **Method:** Human colonic epithelial cells, HT‐29, were incubated with VIP (10^−12^−10^−7^ M). The cells were treated with protein kinase‐A (PK‐A) inhibitors (H‐89, H‐9) or chloride channel‐blockers (diphenylamine‐2‐carboxylate (DPC), 5‐nitro‐2‐(3‐phenylpropylamino) benzoic acid (NPPD)). The expression of AQP3 mRNA and protein was determined by Northern blot and Western blot, respectively. The DNA‐binding activities of cyclic adenosine monophosphate (cAMP) response elements/activating transcription factor (CRE/ATF)) in the nuclear extract were determined by electrophoretic mobility shift assay. **

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Author

AKIHIKO ITOH; TOMOYUKI TSUJIKAWA; YOSHIHIDE FUJIYAMA; TADAO BAMBA

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0815-9319)

Published

2003

Language

EN

Field

Medicine (Health Sciences)