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Can I read Differential roles of Fc gamma RII and Fc gamma RIII in immune complex stimulation of human neutrophils. on EtoBox?
Differential roles of Fc gamma RII and Fc gamma RIII in immune complex stimulation of human neutrophils. by B.A. Brunkhorst; G Strohmeier; K Lazzari; G Weil; D Melnick; H.B. Fleit; E.R. Simons is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Differential roles of Fc gamma RII and Fc gamma RIII in immune complex stimulation of human neutrophils. about?
Insoluble immune complexes (IIC) stimulate human neutrophils through Fc gamma receptors. Freshly isolated human neutrophils express two FcR subclasses, FcRII and FcRIII. We explored the role of FcRII and FcRIII in this activation process by selectively binding each FcR subclass with the Fab fragments of the respective anti-FcR monoclonal antibodies (MFab) before exposure to IIC. Correlation among liganded FcR subclass, IIC binding, and ensuant IIC stimulation was achieved with multiparameter flow cytometry. We utilized rhodamine-labeled anti-FcRIII and fluorescein-labeled IIC to study binding and observed the change in [Ca2+]i in the same cell with a Ca2+ indicator, Indo-1. Treatment with either anti-FcRII (IV.3) or anti-FcRIII (3G8) MFab decreased both the fraction of cells exhibiting a Ca2+ transient and the magnitude of that transient, although only anti-FcRIII but not anti-FcRII significantly inhibited the subsequent IIC binding. In addition, cells treated with anti-FcRII and then stimulated with IIC exhibited a decrease in both the intracellular Ca2+ transient and the later Ca2+ influx, whereas anti-FcRIII totally abolished the mobilization of intracellular Ca2+ without affect
Who reads Differential roles of Fc gamma RII and Fc gamma RIII in immune complex stimulation of human neutrophils.?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- B.A. Brunkhorst; G Strohmeier; K Lazzari; G Weil; D Melnick; H.B. Fleit; E.R. Simons
- Publisher
- Elsevier BV
- Published
- 1992
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)