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Can I read Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy on EtoBox?
Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy by Oliver Kooistra; Lars Herfurth; Edeltraud Lüneberg; Matthias Frosch; Thomas Peters; Ulrich Zähringer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy about?
Two modifications of 5‐acetimidoylamino‐7‐acetamido‐3,5,7,9‐tetradeoxy‐d‐__glycero__‐d‐__galacto__‐non‐2‐ulosonic acid (5‐__N__‐acetimidoyl‐7‐__N__‐acetyllegionaminic acid) in the O‐chain polysaccharide (OPS) of the __Legionella pneumophila__ serogroup 1 lipopolysaccharide (LPS) concern N‐methylation of the 5‐N‐acetimidoyl group in legionaminic acid. Both N‐methylated substituents, the (__N__,__N__‐dimethylacetimidoyl) amino and acetimidoyl(__N__‐methyl)amino group, could be allocated to one single legionaminic acid residue in the long‐ and middle‐chain OPS, respectively. Using mutants devoid of N‐methylated legionaminic acid derivatives, it could be shown that N‐methylation of legionaminic acid correlated with the expression of the mAb 2625 epitope. In the present study we investigated the binding of the LPS‐specific monoclonal antibody mAb 2625 to isolated OPS with surface‐plasmon‐resonance biomolecular interaction analysis and saturation‐transfer‐difference (STD) NMR spectroscopy in order to map the mAb 2625 epitope on a molecular level. It could be demonstrated that the binding affinity of the N‐methylated legionaminic acid derivatives was independent from the size of the isola
Who reads Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Oliver Kooistra; Lars Herfurth; Edeltraud Lüneberg; Matthias Frosch; Thomas Peters; Ulrich Zähringer
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)