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Can I read Cultures from adult rat liver cells I. Establishment of monolayer cell-cultures from normal liver on EtoBox?
Cultures from adult rat liver cells I. Establishment of monolayer cell-cultures from normal liver by P. T. Iype is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cultures from adult rat liver cells I. Establishment of monolayer cell-cultures from normal liver about?
Establishment of monolayer cell-cultures from adult normal rat liver is described using cells isolated by a collagenase-hyaluronidase perfusion technique. The cells plated originally were polygonal in shape and contained a large number of mitochondria. However, cells with less granules were also observed in the primary culture, after a few days of plating. In subsequent subcultures, the latter type of cells could be grown continuously. After an initial lag, the cells attained a doubling time of 55 hours and a plating efficiency of 20%. The cells are "normal" with respect to their karyology, morphology and growth pattern. Difficulties encountered during the culture and the possible uses of these cultured cells in studies on carcinogenesis in vitro are discussed.
Who reads Cultures from adult rat liver cells I. Establishment of monolayer cell-cultures from normal liver?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- P. T. Iype
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 1971
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)