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Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field by Barbara Stopa; Barbara Piekarska; Leszek Konieczny; Marcin Król; Janina Rybarska; Anna Jagusiak; Paweł Spólnik; Irena Roterman; Barbara Urbanowicz; Piotr Piwowar; Krzysztof Lewiński is a Chemistry article available to read on EtoBox.
What is Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field about?
This study describes a technique which makes it possible to introduce the amyloid-like order to protein aggregates by using the scaffolding framework built from supramolecular, fibrillar Congo red structures arranged in an electric field. The electric field was used not only to obtain a uniform orientation of the charged dye fibrils, but also to make the fibrils long, compact and rigid due to the delocalization of pi electrons, which favors ring stacking and, as a consequence, results in an increased tendency to self-assemble. The protein molecules (immunoglobulin L chain lambda, ferritin) attached to this easily adsorbing dye framework assume its ordered structure. The complex precipitating as plate-like fragments shows birefringence in polarized light. The parallel organization of fibrils can be observed with an electron microscope. The dye framework may be removed via reduction with sodium dithionite, leaving the aggregated protein molecules in the ordered state, as confirmed by X-ray diffraction studies.
Who reads Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Barbara Stopa; Barbara Piekarska; Leszek Konieczny; Marcin Król; Janina Rybarska; Anna Jagusiak; Paweł Spólnik; Irena Roterman; Barbara Urbanowicz; Piotr Piwowar; Krzysztof Lewiński
- Publisher
- Walter de Gruyter GmbH
- Published
- 2010
- Language
- EN
- Field
- Chemistry (Physical Sciences)