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Developmental appearance, antigenic profile, and proliferation of glial cells of the human embryonic spinal cord: An immunocytochemical study using dissociated cultured cells by Dr. Francesca Aloisi; Adele Giampaolo; Giovanni Russo; Cesare Peschle; Giulio Levi is a Neuroscience article available to read on EtoBox.

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## Abstract We have investigated the time of appearance of the earliest differentiating glial cell types of human spinal cord using a panel of antigenic markers to identify them in cultures from 6‐ to 9‐week‐old human embryos. Immunolabeling performed at 14 h in vitro with the O4 mAb, an early oligodendrocyte marker, showed the presence of oligodendrocytes during the 7th week of age. At 8 weeks only a few of the O4^+^ cells expressed galactocerebroside (GalC), a marker of more differentiated oligodendrocytes. All the O4^+^ and GalC^+^ cells were vimentin^+^ and some of the GalC^+^ cells were A2B5^+^, GD3^+^ and SSEA‐1^+^. During the first week in vitro many of the O4^+^ cells exhibiting a more immature, bi‐ or tri‐polar morphology incorporated [^3^H]thymidine into their nuclei. Cells expressing the astrocyte‐specific marker GFAP could be first observed at 8 weeks; almost all of these GFAP^+^ cells, which should correspond to radial glia on the basis of the current literature, were vimentin^+^, A2B5^+^, GD3^+^, and SSEA‐1^+^. At 2 days in vitro incorporation of [^3^H]thymidine could be shown in a small fraction of these cells. The finding that radial glia and oligodendrocytes expres

Who reads Developmental appearance, antigenic profile, and proliferation of glial cells of the human embryonic spinal cord: An immunocytochemical study using dissociated cultured cells?

Author

Dr. Francesca Aloisi; Adele Giampaolo; Giovanni Russo; Cesare Peschle; Giulio Levi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

1992

Language

EN

Field

Neuroscience (Life Sciences)