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N-methyl-D-aspartate-evoked release of cyclo-oxygenase products in rabbit hippocampus: An in vivo microdialysis study by J. W. ?azarewicz; E. Sali?ska is a Neuroscience article available to read on EtoBox.

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In vivo microdialysis of the rabbit hippocampus was used to study the effects of N-methyl-D-aspartate (NMDA) receptor stimulation on dialysate concentrations of thromboxane B, (Tx B2)and 6-keto prostaglandin F1, (6-keto PGF,,)-immunoreactive materials that are stable metabolites of biologically active thromboxane A, and prostacyclin. All pharmacological substances were applied in the dialysis medium. The application of 1 mM NMDA for 20 min resulted in five-and eightfold increases in Tx B, and 6-keto PGF,, concentrations, respectively. An increase in NMDA concentration to 2.5 mM did not potentiate a peak eicosanoid release, but significantly prolonged this effect. Either 10 pM MK-801 or the extrusion of Ca2+ from the dialysis medium inhibited the release by about 50%. Quinacrine, a phospholipase A, inhibitor (250 pM), decreased the NMDA-evoked eicosanoid release by 30%, whereas 10 pM indomethacin, a cyclo-oxygenase inhibitor, completely suppressed the release. One hundred micromolar furegrelate, an inhibitor of thromboxane synthase, reduced by 75% Tx B, release with concomitant 100% increase in 6-keto PGF,, formation. Thus, stimulation of NMDA receptors induces calcium-dependent for

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Author

J. W. ?azarewicz; E. Sali?ska

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

1995

Language

EN

Field

Neuroscience (Life Sciences)