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Regulation of levels of IL-1 mRNA in human fibroblasts by Kenji Yamato; Ziad El-Hajjaoui; H. Phillip Koeffler is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Regulation of levels of IL-1 mRNA in human fibroblasts about?

interleukin-1 (IL-1) has a crucial role in host defenses, inflammatory processes, and tissue homeostasis. A wide variety of cells produce this protein in response to a number of extracellular stimuli including microorganisms, antigenic stimuli, and products from other cells. Regulation of IL-1 production at the molecular level is poorly understood. We studied expression, intracellular signals, and posttranscriptional regulation of IL-1 mRNA in human mesenchymal cells by using Northern blot analysis. Tumor necrosis factor alpha (TNFa) and activators of protein kinase C including 12-0-tetradecanoylphorbol-13-acetate (TPA) and teleocidin induced the accumulation of IL-l p mRNA in human fibroblasts (WI-38). Effect of TNFa was not blocked by inhibitors of either protein synthesis (cycloheximide) or protein kinase C activity. Accumulation of IL-l p mRNA was also increased by a calcium ionophore (A23187) and an inhibitor of the N a f / K f pump (ouabain); both compounds are known to increase cytoplasmic levels of C a + + . Stability of I L -l P mRNA in fibroblasts exposed to TPA was more than fourfold greater than after fibroblasts were exposed to either TNFa or cycloheximide. This sugges

Who reads Regulation of levels of IL-1 mRNA in human fibroblasts?

Author

Kenji Yamato; Ziad El-Hajjaoui; H. Phillip Koeffler

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)