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Biodistribution and pharmacokinetics of ^125^I‐labeled monoclonal antibody M75 specific for carbonic anhydrase IX, an intrinsic marker of hypoxia, in nude mice xenografted with human colorectal carcinoma by Adrián Chrastina; Jan Závada; Seppo Parkkila; Štefan Kaluz; Milota Kaluzová; Július Rajčáni; Jaromír Pastorek; Silvia Pastoreková is a Medicine article available to read on EtoBox.
What is Biodistribution and pharmacokinetics of ^125^I‐labeled monoclonal antibody M75 specific for carbonic anhydrase IX, an intrinsic marker of hypoxia, in nude mice xenografted with human colorectal carcinoma about?
## Abstract Carbonic anhydrase IX (CA IX) is frequently expressed in human carcinomas and absent from the corresponding normal tissues. Strong induction by tumor hypoxia predisposes CA IX to serve as a target for cancer diagnostics and therapy. Here we evaluated targeting properties and pharmacokinetics of CA IX‐specific monoclonal antibody (MAb) M75. Binding parameters of ^125^I‐labeled M75, including equilibrium dissociation constant, hypoxia‐related binding to various cell lines and internalization, were analyzed __in vitro__. Biodistribution of ^125^I‐M75 in nude mice bearing HT‐29 human colorectal carcinoma xenografts with hypoxic pattern of CA IX expression was studied by measurements of radioactivity in dissected tissues and macroautoradiography of tissue sections. Pharmacokinetics of intravenously administered ^125^I‐M75 was described using a 2‐compartment model. Blood clearance showed a distribution phase t~1/2~(α) = 3.4 hr and an elimination phase t~1/2~(β) = 55.3 hr postinjection. Despite predominant CA IX localization in less accessible perinecrotic regions, ^125^I‐M75 exhibited specific accumulation in xenograft, with a mean uptake of 15.3 ± 3.6% of injected dose per g
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- Author
- Adrián Chrastina; Jan Závada; Seppo Parkkila; Štefan Kaluz; Milota Kaluzová; Július Rajčáni; Jaromír Pastorek; Silvia Pastoreková
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)