Opening book details…
Can I read Identification of the epitope for anti‐cystatin C antibody on EtoBox?
Identification of the epitope for anti‐cystatin C antibody by Anna Śladewska; Aneta Szymańska; Marlena Kordalska; Agnieszka Lewandowska; Aleksandra S. Kołodziejczyk; Gabriela Paraschiv; Michael Przybylski; Paulina Czaplewska is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Identification of the epitope for anti‐cystatin C antibody about?
## Abstract Human cystatin C (hCC), like many other amyloidogenic proteins, has been shown to form dimers by exchange of subdomains of the monomeric protein. Considering the model of hCC fibrillogenesis by propagated domain swapping, it seems possible that inhibition of this process should also suppress the entire process of dimerization and fibrillogenesis which leads to specific amyloidosis (hereditary cystatin C amyloid angiopathy (HCCAA)). It was reported that exogenous agents like monoclonal antibody against cystatin C are able to suppress formation of cystatin C dimers. In the effort to find a way of controlling the cystatin fibrillization process, the interactions between monoclonal antibody Cyst‐13 and cystatin C were studied in detail. The present work describes the determination of the epitope of hCC to a monoclonal antibody raised against cystatin C, Cyst‐13, by MALDI mass spectrometry, using proteolytic excision of the immune complex. The shortest epitope sequence was determined as hCC(107‐114). Affinity studies of synthetic peptides revealed that the octapeptide with epitope sequence does not have binding ability to Cyst‐13, whereas its longer counterpart, hCC(105–114)
Who reads Identification of the epitope for anti‐cystatin C antibody?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Anna Śladewska; Aneta Szymańska; Marlena Kordalska; Agnieszka Lewandowska; Aleksandra S. Kołodziejczyk; Gabriela Paraschiv; Michael Przybylski; Paulina Czaplewska
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0952-3499)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)