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Interesting probiotic traits of mother's milk Lactobacillus isolates; from bacteriocin to inflammatory bowel disease improvement by Milad Abdi; Vahid Lohrasbi; Arezoo Asadi; Maryam Esghaei; Faramarz Masjedian Jazi; Mahdi Rohani; Malihe Talebi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Aims and background: Lactobacillus spp. are an important element in breast milk. this component has a beneficial effect on the composition of the intestinal microflora and the intestinal immune system. the aim of this study was to isolate and identify lactobacillus strains in breast milk and evaluate some of their probiotic properties, such as presence of bacteriocin genes, adhesion to ht-29 cell line, competition with enteropathogens in cell culture, and effect on serum level of lipids and digestive enzymes, and mice model of inflammatory bowel disease (ibd). ## Materials and methods: A total of 323 lactic acid bacteria (lab) were isolated from breast milk samples of healthy mothers with the age ranges from 21 to 45 years old. these isolates were subjected to phenotypic and molecular experiments. the frequency of bacteriocin genes was determined by polymerase chain reaction (pcr). adhesion of lactobacillus isolates to ht-29 cells was measured based on the number of attached bacterial cells in 20 fields of the light microscopy. competition test was done by colony count and real-time pcr procedures. five strongly adhesive lactobacillus strains were selected and administered orall

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It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Milad Abdi; Vahid Lohrasbi; Arezoo Asadi; Maryam Esghaei; Faramarz Masjedian Jazi; Mahdi Rohani; Malihe Talebi
Publisher
Elsevier BV
Published
2021
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)

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