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Extracellular Recordings from Locally Dense Microelectrode Arrays Coupled to Dissociated Cortical Cultures by L. Berdondini; P. Massobrio; M. Chiappalone; M. Tedesco; K. Imfeld; A. Maccione; M. Gandolfo; M. Koudelka-Hep; S. Martinoia is a Neuroscience article available to read on EtoBox.
What is Extracellular Recordings from Locally Dense Microelectrode Arrays Coupled to Dissociated Cortical Cultures about?
High-density microelectrode arrays (MEAs) enabled by recent developments of microelectronic circuits (CMOS-MEA) and providing spatial resolutions down to the cellular level open the perspective to access simultaneously local and overall neuronal network activities expressed by in vitro preparations. The short inter-electrode separation results in a gain of information on the micro-circuit neuronal dynamics and signal propagation, but requires the careful evaluation of the time resolution as well as the assessment of possible cross-talk artifacts. In this respect, we have realized and tested Pt high-density (HD)-MEAs featuring four local areas with 10microm inter-electrode spacing and providing a suitable noise level for the assessment of the high-density approach. First, simulated results show how possible artifacts (duplicated spikes) can be theoretically observed on nearby microelectrodes only for very high-shunt resistance values (e.g. R(sh)=50 kOmega generates up to 60% of false positives). This limiting condition is not compatible with typical experimental conditions (i.e. dense but not confluent cultures). Experiments performed on spontaneously active cortical neuronal networ
Who reads Extracellular Recordings from Locally Dense Microelectrode Arrays Coupled to Dissociated Cortical Cultures?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- L. Berdondini; P. Massobrio; M. Chiappalone; M. Tedesco; K. Imfeld; A. Maccione; M. Gandolfo; M. Koudelka-Hep; S. Martinoia
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-0270)
- Published
- 2009
- Language
- EN
- Field
- Neuroscience (Life Sciences)