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Use of S-adenosylmethionine as an index of methionine recycling in rat liver slices by Anthony J. Barak; Harriet C. Beckenhauer; Dean J. Tuma is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A simplified method for the measurement of methionine recycling in liver slices was developed. The method, a double-labeling procedure, utilizes S-adenosylmethionine as an index of recycling and offers several advantages over methionine, which has been used in other laboratories to determine methionine recycling in plants and intact animals. These advantages include (i) ease of isolation of index substance from tissue samples; (ii) elimination of oxidation problems prevalent with use of methionine; (iii) need for less initial concentrations of [methyl-'H] methionine and ["Slmethionine used as tracers in the measurement of methionine recycling.

Who reads Use of S-adenosylmethionine as an index of methionine recycling in rat liver slices?

Author

Anthony J. Barak; Harriet C. Beckenhauer; Dean J. Tuma

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1982

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)