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High-performance Liquid Chromatography Detection of Morphine by Fluorescence After Post-column Derivatisation by Peter E. Nelson; Susan L. Nolan; Keith R. Bedford is a Chemistry article available to read on EtoBox.

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A reversed-phase high-performance liquid chromatographic method for the fluorometric determination of morphine in biological samples has been developed. ## Column et'fluent is mixed with alkaline potassium ferricyanide to produce the tluorescent dimer pseudomorphine_ The method provides higher chromatogaphic specificity and sensitivity than conventional high-performance liquid chromatography-IMorphine can be detected at levels of 10 nS on column. INTRODLXTION ## Fluorescent derivatisation for enhanced detection and selectivity has been in use in chromatography for many years, and the availability of fluorescence detection systems for liquid chromatography systems has allowed the application of fluorescent derivatisation to that field. Derivatives may be formed before the sample is introduced (pre-column) or between the column outlet and the detector (post-column). Pre-column derivatisation is necessary if the reaction is slow, but post-column derivatisation is less likely to form artifacts, allows the simultaneous use of different detection systems, e-g. series ultraviolet (UV) detection and fluorescence detection\_ and does not alter the chromatographic properties of the compounds

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Author

Peter E. Nelson; Susan L. Nolan; Keith R. Bedford

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1982

Language

EN

Field

Chemistry (Physical Sciences)