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This document provides instructions for a recombinant DNA workshop. It includes a DNA sequence and questions about restriction enzyme digestion of that sequence using EcoRI, HindIII, BamHI, XbaI and SmaI. Students are asked to identify which enzymes could extract genes from the sequence without fragmentation, determine fragment sizes, design a polylinker for cloning a gene fragment, and predict fragment patterns from single or double digests on an agarose gel.

Author

SANTIAGO AVILA DUQUE

Language

EN