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A large conductance [Ca^2+^]~i~‐independent K^+^ channel expressed in HaCaT keratinocytes by V. H. Nguyen; F. Markwardt is a Medicine article available to read on EtoBox.

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**Abstract:** Patch‐clamp recordings were carried out in the inside‐out configuration in human keratinocytes of the cell line HaCaT. Patch pipettes were filled with 150 mM KCl, 1 mM CaCl~2~ and 10 mM HEPES. In symmetrical KCl solutions, single channel currents from a large conductance channel (about 170 pS) were measured. Replacement of 120 mM KCl by K‐aspartate had only a minor influence on the single channel conductance and on the reversal potential. In intracellular solution in which K^+^ has been replaced by Na^+^ or NMDG^+^, the reversal potential shifted to >+ 40 mV indicating K^+^ as the main charge carrier. The channels were neither dependent on intracellular Ca^2+^ (between 0.8 nM and 10 μM), ATP (at 0 and 1 mM) nor Mg^2+^ (at 0 and 0.5 mM). The mean current showed an outward rectification that can be mainly attributed to the voltage dependence of the open probability. The channels displayed bursting kinetics with a mean open time of about 2 ms and closed times of about 0.2, 2 and 20 ms. The mean open probability was usually low (0.05) but increased occasionally (0.6) mainly due to a lower probability of long closings. We conclude that these K^+^ channels contribute to the

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Author

V. H. Nguyen; F. Markwardt

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0906-6705)

Published

2002

Language

EN

Field

Medicine (Health Sciences)