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UDP-galactose 4-epimerase: a key enzyme in exopolysaccharide formation by Lactobacillus casei CRL 87 in controlled pH batch cultures by F. Mozzi; G. Savoy de Giori; G. Font de Valdez is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Aims: To evaluate the relationship between exopolysaccharide (eps) production and the sugar nucleotide biosynthetic enzymes in lactobacillus casei crl 87 under optimum growth conditions for polymer formation: controlled ph on galactose or glucose. studies with an eps mutant were carried out to determine the key enzymes in eps synthesis under the above culture conditions. ## Methods and results: Eps concentration was estimated by the phenol/sulphuric acid method, while the activities of the biosynthetic enzymes were determined spectrophotometrically by measuring the formation or disappearance of nad(p)h at 340 nm. an environmental ph of 5.0, using galactose as carbon source, markedly improved not only polymer production and yield but also, cell growth and lactic acid production. analysis of the activities of the eps precursor-forming enzymes revealed that polysaccharide synthesis was correlated with uridine-diphosphate (udp)-glucose pyrophosphorylase and udp-galactose 4-epimerase under these growth conditions. ## Conclusions: Eps synthesis by lact. casei crl 87 was considerably improved at a controlled ph of 5.0 with galactose as carbon source, and was correlated with the activit

Who reads UDP-galactose 4-epimerase: a key enzyme in exopolysaccharide formation by Lactobacillus casei CRL 87 in controlled pH batch cultures?

Author

F. Mozzi; G. Savoy de Giori; G. Font de Valdez

Publisher

Oxford University Press (OUP)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)